Beacon: 20-0011: 2,4-Dichlorophenoxyacetic acid (2,4-D)in Water by Immunoassay, Microtiter Plate
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Official Method Name
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2,4-D Plate Kit |
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Current Revision
| 2010 |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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2,4-D in the sample competes with 2,4-D-enzyme conjugate to bind to antibodies which are immobilized on the microtiter plate. In the assay procedure you will: - Add a sample containing 2,4-D to a test well, followed by 2,4-D-enzyme conjugate. The conjugate competes with any 2,4-D in the sample for the same antibody binding sites. - Wash away any unbound molecules after 60 minutes of incubation. - Add clear substrate solution to each well. In the presence of bound 2,4-D-enzyme conjugate, the colorless substrate is converted to a blue compound. - Add stop solution to terminate the reaction. - Read the color intensity at 450 nm. - Calculate the concentration of 2,4-D in the sample by comparing its absorbance to the absorbance of calibrators. |
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Scope and Application
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This method determines 2,4-D residues in water. |
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Applicable Concentration Range
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2.0 - 200 |
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Interferences
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Cross reactivity: 2,4-D (100%), 2,4-D-methyl ester (400%),2,4-DB (100%), 2,4-D-isopropyl ester (67%), 2,4-DB-butyl ester (53%), 2,4,5-T (9.5%), MCPA (9.3%), Dichlorprop (2.7%), 2,4,5-TP (2.2%). |
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Quality Control Requirements
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Sample Handling
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Any particles in water sample should be removed by filtration or centrifugation. |
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Maximum Holding Time
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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