Abraxis: 515565:  Cotinine by Immunoassay, Microtiter Plate

  • Summary
  • Analytes
  • Revision
  • Data and Sites
Official Method Name
Abraxis Cotinine ELISA Plate Kit PN 515565
Current Revision
2008
Media
WATER
Instrumentation
Immunoassay
Method Subcategory
Organic
Method Source
  Abraxis
Citation
  Abraxis User Guides and Flowcharts
Brief Method Summary
Cotinine is detected using a colorimetric immunoassay (ELISA) procedure. A sample (0.05 mL), enzyme conjugate (Cotinine-HRP), and an antibody solution against Cotinine are added to plate wells pre-coated with polyclonal antibodies (goat anti-rabbit). Both the Cotinine (if present) in the sample and Cotinine-HRP conjugate compete to bind to the antibody solution in proportion to their concentrations. The anti-Cotinine antibodies are then bound to the immobilized polyclonal antibodies on the plate. After an incubation, the unbound molecules are washed and decanted. A substrate is then added which is catalyzed by the enzyme and converted from a colorless to a blue solution. The reaction is terminated with the addition of a dilute acid. The concentration of Cotinine in the sample is determined by measuring its absorbance at a specific wavelength (450nm) using a photometer and comparing its absorbance to the absorbance of the calibrators.
Scope and Application
This method determines Cotinine in water (groundwater, surface water, well water) which is an indicator of human source pollution.
Applicable Concentration Range
0.045-5.0 ng/mL
Interferences
Cross-reactivity: High concentrations of Nicotine may produce false positive responses for Cotinine.
Quality Control Requirements
(A) Calibration with 5 standards and 1 blank, all analyzed in duplicate. (B) Precision: 3 matrix samples with different levels in the range for quantitative analysis analyzed daily for 3 days with 5 replicates in each of 5 assays. (C) Accuracy: 5 matrix samples spiked with the target analyte at 3 different levels in the range for quantitative analysis. (D) Validation: Analysis of 4 positive and 4 negative samples by an independent method, for confirmation.
Sample Handling
Samples are collected in glass containers with Teflon-lined caps; for compliance monitoring, samples should be held no longer than 14 days. If samples are held frozen, they can be held for longer periods of time. Immunoassay reagents are stored refrigerated until use. Samples containing gross particulate matter should be filtered using a 1 micron filter or centrifuged before use. Drinking water samples are typically dechlorinated with 0.008% sodium thiosulfate.
Maximum Holding Time
14 days at 4 degrees C, longer if frozen
Relative Cost
Less than $50
Sample Preparation Methods