Standard Methods: 9213B: Staphylococcus aureus in water by Tube Culture
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Official Method Name
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9213 Recreational Waters |
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Current Revision
| Standard Methods Online |
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Media
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WATER |
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Instrumentation
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Tube Culture |
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Method Subcategory
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Microbiological |
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Method Source
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Citation
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Brief Method Summary
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Inoculate tubes of M-staphylococcus broth and incubate at 35 deg. C. for 24 hours. Hold the original enrichment sample but streak from positive (turbid) tubes on plates of lipovitellenin-salt mannitol agar and incubutate at 35 deg. C. for 48 hours. Opaque yellow zones around colonies indicate positive reactions. Refer to Section 7 for additional procedures if the plate is negative. |
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Scope and Application
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This method is used for analysis of swimming pool water. |
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Applicable Concentration Range
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Interferences
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None specified |
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Quality Control Requirements
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None specified |
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Sample Handling
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Collect samples as directed in Section 9060A. Use sterile containers of 120 to 480 mL and add sufficient sodium thiosulfate to provide a concentration of about 100 mg/L in the sample. |
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Maximum Holding Time
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Analyze samples as soon as possible after collection as per Section 9060B. |
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Relative Cost
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Unknown |
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Sample Preparation Methods
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