EPA-OW: Test Method 1000.0: Larval Survival and Growth Test using Fathead Minnow

Summary
Analytes
Revision
Data and Sites

Official Method Name

EPA Test Method 1000.1: Fathead Minnow, Pimephales promelas, Larval Survival and Growth Test Method

Current Revision

Fourth Edition, October 2002

Media

WATER

Instrumentation

Toxicity

Method Subcategory

Chronic Toxicity

Method Source

EPA-OW

Citation

Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms

Brief Method Summary

Fathead Minnow, Pimephales promelas, larvae are exposed in a static renewal system for seven days to different concentrations of effluent or to receiving water. Test results are based on the survival and weight of the larvae.

Scope and Application

This method estimates the chronic toxicity of effluents and receiving water to the fathead minnow, Pimephales promelas, using newly hatched larvae in a seven-day, static renewal test. The effects include the synergistic, atagonistic, and additive effects of all the chemical, physical, and biological components which adversely affect the physiological and biochemical functions of the test organisms.

Applicable Concentration Range

Effluents: 5 and a control

Receiving waters: 100% receiving water and a control (recommended)

Interferences

Introduced toxic substances; Low D.O.; High concentrations of suspended and/or dissolved solids; Pathogenic and/or predatory organisms;

Quality Control Requirements

Reference toxicant testing with control charts for each combination of toxicant, test species, test condition, and endpoint.

Sample Handling

It is recommended that samples be held at 0 - 6degC until used.

Maximum Holding Time

36h

Relative Cost

Unknown

Sample Preparation Methods

Filtration, Dechlorination.

This method has 2 analytes associated with it.

Analyte	Detection Level	Bias	Precision	Pct False Positive	Pct False Negative	Spiking Level
Growth/Biomass(X00009) Biomass/Growth Growth/Biomass	N/A	N/A	N/A
Survival(X00008) Survival	N/A	N/A	N/A

Precision Descriptor Notes:	It is a laboratory's responsibility to demonstrate its ability to obtain consistent, precise results with reference toxicants before it performs toxicity tests with effluents for permit compliance purposes. To meet this requirement, the intra-laboratory precision, expressed as percent coefficient of variation, of each type of test to be used in a laboratory should be determined by performing five or more tests with different batches of test organisms using the same reference toxicant, at the same concentrations, with the same test conditions, and the same data analysis methods.
Detection Level Note:	N/A

Precision Descriptor Notes:

It is a laboratory's responsibility to demonstrate its ability to obtain consistent, precise results with reference toxicants before it performs toxicity tests with effluents for permit compliance purposes. To meet this requirement, the intra-laboratory precision, expressed as percent coefficient of variation, of each type of test to be used in a laboratory should be determined by performing five or more tests with different batches of test organisms using the same reference toxicant, at the same concentrations, with the same test conditions, and the same data analysis methods.

Detection Level Note:

N/A

Revision	PDF/Link
Fourth Edition, October 2002

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EPA-OW: Test Method 1000.0: Larval Survival and Growth Test using Fathead Minnow

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