MD DNR: MD DNR MBSS: Benthic Sampling Protocols for Maryland Streams
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Official Method Name
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Maryland Biological Stream Survey Sampling Manual - Benthic Sampling Protocols |
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Current Revision
| 2001 |
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Media
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WATER (Waterbody type - Wadeable stream) |
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Instrumentation
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Invertebrate Net (w/ handle) |
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Method Subcategory
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Population/Community |
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Method Source
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Citation
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Brief Method Summary
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A combination of habitats supporting the most diverse macroinvertebrate community within a sample segment is sampled qualitatively (multihabitat). Sampling begins by surveying the 75 meter segment to locate the most productive habitat. When sampling riffles, the net (600-micron mesh D-net) is placed on the downstream edge, and the substrate is disturbed down to the hardpan (5 to 8 cm). Larger particles are rubbed by hand to remove organisms. This process is repeated on the upstream edge until 20 square feet of substrate has been disturbed. If only log and snag substrates are available then rub off by hand 20 square feet of surface area. When sampling habitats other than riffles and snags, the D-net should be used in a jabbing/sweeping motion until 20 square feet has been sampled. The sample is then washed into a sieve bucket, the large debris is cleaned and removed, and the sample is preserved and labeled (internal/external) in a sample bucket with 70% ethanol. |
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Scope and Application
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One objective of the Maryland Biological Stream Survey (MBSS) is to assess with known confidence the current status of the biological resources in non-tidal streams and rivers in Maryland. Biological resources are evaluated on a local, regional, and statewide basis using two endpoints, fishability and biological integrity. Another objective of the MBSS is to monitor indicators of pollution exposure and habitat condition to identify local, regional, or statewide causes of adverse effects, including acid deposition point source discharges, and others. Additional objectives of the survey are to provide an inventory of biodiversity in Marylands streams and rivers and a means to focus protection and restoration activities. Information from the MBSS is used to provide statistical summaries and interpretive reports on ecological status and fishability to decision-makers and the public. |
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Applicable Concentration Range
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N/A |
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Interferences
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None provided. |
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Quality Control Requirements
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To be effective, the QA program must continually monitor the accuracy, precision, completeness, comparability, and representativeness of the data during all phases of the program. Components of the MBSS field QA program include: thorough investigator training; development of and adherence to project protocols and guidelines; comprehensive field and laboratory data documentation and management; verification of data reproducibility; and instrument calibration. |
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Sample Handling
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Preserve in 70% ethanol |
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Maximum Holding Time
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Indefinite with proper preservation. |
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Relative Cost
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3 - 6 hrs. per site/sample |
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Sample Preparation Methods
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Rinse and discard large material. Labels |
