EPA-OW: Test Method 2019.0: Acute Toxicity Tests for Waters Using Rainbow Trout and Brook Trout
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Official Method Name
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EPA Test Method 20019.0: Rainbow Trout, Oncorhynchus mykiss, and Brook Trout, Salvelinus fontinalis, Acute Toxicity Tests with Effluents and Receiving Waters |
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Current Revision
| Fifth Edition, October 2002 |
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Media
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WATER |
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Instrumentation
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Organism: Refer to method for specific species and genus |
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Method Subcategory
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Acute Toxicity |
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Method Source
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Citation
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Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms. Fifth Edition, October 2002. EPA Volume 821-R-02-012 |
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Brief Method Summary
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Early life stage Oncorhynchus mykiss and Salvelinus fontinalis are exposed to varying concentrations of effluent or receiving water in a static non-renewal, static-renewal, or flow-through system for a pre-determined amount of time (24, 48, or 96 h). The survival in each concentration is compared to the survival in the control to determine the concentration of effluent or receiving water lethal to 50% of test organisms. |
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Scope and Application
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This procedure describes an acute toxicity test method for use in the National Pollutant Discharge Elimination System (NPDES) Permits Program to identify effluents and receiving waters containing toxic materials in acutely toxic concentrations. This method is also suitable for determining the toxicity of specific compounds contained in discharges. This test may be conducted in a central laboratory or on-site, by the regulatory agency or the permittee. |
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Applicable Concentration Range
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Effluents: 5 and a control (minimum) Receiving Water: 100% receiving water and a control (recommended) |
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Interferences
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N/A |
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Quality Control Requirements
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Routine reference toxicant testing |
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Sample Handling
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Samples should be held at 0-6 deg. C prior to testing |
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Maximum Holding Time
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36h |
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Relative Cost
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2 - 5 days to complete test |
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Sample Preparation Methods
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Coarse filtration; Dechlorination |
