EPA-OW: Test Method 1004.0: Larval Survival and Growth Test using Sheepshead Minnow
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Official Method Name
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EPA Test Method 1004.0, Sheepshead Minnow, Cyprinodon variegatus, Larval Survival and Growth Test Method. |
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Current Revision
| Third Edition, October 2002 |
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Media
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WATER |
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Instrumentation
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Toxicity |
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Method Subcategory
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Chronic Toxicity |
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Method Source
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Citation
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Brief Method Summary
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Sheepshead Minnow, Cyprinodon variegatus, larvae (preferably less than 24h old) are exposed in a static renewal system for seven days to different concentrations of effluent or to receiving water. Test results are based on the survival and weight of the larvae. |
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Scope and Application
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This method, adapted in part from USEPA (1987b), estimates the chronic toxicity of effluents and receiving waters to the sheepshead minnow, Cyprinodon variegatus, using newly hatched larvae in a seven-day, static-renewal test. The effects include the synergistic, antagonistic, and additive effects of all the chemical, physical, and biological components which adversely affect the physiological and biochemical functions of the test species. |
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Applicable Concentration Range
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Effluent: 5 and control Receiving water: 100% receiving water (or minimum of 5) and a control |
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Interferences
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Introduced toxic substances from testing equipment; Low D.O., high concentration of dissolved or suspended solids; Extremes of pH; Pathogenic or predatory organisms |
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Quality Control Requirements
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Acceptable side-by-side reference toxicant testing; 80% or greater survival in controls, with a 0.50 mg average dry weight at conclusion of test. |
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Sample Handling
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Samples must be stored at 0 - 6 deg. C prior to use. 1. Adjust sample salinity to appropriate level. 2. Sample may need to be coarse-filtered to remove suspended solids. 3. Sample may need to be warmed slowly to test temp. 4. Dechlorination |
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Maximum Holding Time
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Sample must be used within 24h |
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Relative Cost
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Greater than 5 days to complete test |
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Sample Preparation Methods
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Above |
