ORNL: ORNL-01:  Toxins in Water by Chlorophyll Fluorescence

  • Summary
  • Analytes
  • Revision
  • Data and Sites
Official Method Name
AquaSentinel - WaterSentry chlorophyll fluorescence
Current Revision
2005
Media
WATER
Instrumentation
Fluorescence Spectroscopy
Method Subcategory
Acute Toxicity
Method Source
  ORNL
Citation
  Greenbaum, E., Rodriguez, M., Jr. Sanders, CA, 2004, AquaSentinel (SM): Biosensors for rapid monitoring of primary-source drinking water. In: Norling, P.M., Wood-Black, F. (eds) Water and Sustainable Development: Opportunities for the Chemical Sciences, CSR Workshop Report Series, National Academy Press, Washington, D.C., 2004.
Brief Method Summary
AquaSentinel provides early warning of chemical toxin and selected biotoxin contamination in primary-source water supplies. It monitors naturally occurring algae and applies the fundamental principles of photosynthesis and state-of-the-art light-measuring instruments to provide continuous, unattended surveillance of both standing and flowing water supplies. This technology can be deployed anywhere in the world drinking water is drawn from sources that are exposed to sunlight. AquaSentinel collects real-time data in the field and sends it by remote encrypted wireless transmissions to a command center. Results are obtained in minutes and can give decision makers hours in which to respond to potentially abnormal conditions.
Scope and Application
This method is designed for early warning of contamination of primary-source drinking water supplies.
Applicable Concentration Range
ppm
Interferences
Chlorine; photoinhibition due to diurnal cycle of the algae (system has been designed to address this interference).
Quality Control Requirements
Recommended minimal maintenance: Monthly.
Sample Handling
Automated and continuous. WaterSentry has two bags for sample collection. Samples can be sealed and stored in the system upon retrieval. Dark adaptation required. In heavily-sedimented streams, filtering may be required.
Maximum Holding Time
See "sample Handling"
Relative Cost
Greater than $400
Sample Preparation Methods
No reagents/chemical methods required.