Standard Methods: 6040D: Solid-Phase Microextraction (SPME)
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Official Method Name
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Solid-Phase Microextraction (SPME) |
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Current Revision
| Standard Methods Online |
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Media
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WATER |
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Instrumentation
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Gas Chromatography with Mass Spectrometry Detection |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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Relies on the adsorption of target compounds on a SPME fiber. The fiber is placed in the headspace above the sample in a closed vessel and allowed to equilibrate with the aqueous sample. After equilibration time, the fiber is removed and inserted directly into the injection port of a GC or GC/MS system. |
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Scope and Application
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Taste- and odor-causing compounds: methyl isoborneol (MIB), geosmin, isopropyl methoxy pyrazine (IPMP) |
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Applicable Concentration Range
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10 to 100 ng/L |
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Interferences
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Any organic compounds present in the sample may be adsorbed onto the fiber and desorb in the GC/MS. The mass spectrum serves to identify the compound of interest and eliminate possible interferences. |
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Quality Control Requirements
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LCS, LFM |
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Sample Handling
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Collect samples in either a single amber glass bottle of at least 250 mL or in two amber glass vials (minimum size 60 mL), preferably with no headspace. Preserve sample with 10 mg mercuric chloride/L sample if it will not be analyzed within 3 d of sampling. Pack samples in ice and ship to laboratory for next-day delivery if they are not analyzed immediately. Store samples under refrigeration. |
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Maximum Holding Time
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Relative Cost
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Greater than $400 |
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Sample Preparation Methods
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