ASTM: D4455: Aquatic Bacteria by Epifluoresence Microscopy
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Official Method Name
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Standard Test Method for Enumeration of Aquatic Bacteria by Epifluorescence Microscopy Counting Procedure |
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Current Revision
| 1985 |
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Media
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WATER |
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Instrumentation
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Epifluorescence Microscopy |
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Method Subcategory
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Microbiological |
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Method Source
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Citation
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Brief Method Summary
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Enumeration of aquatic bacteria is obtained by passing a water sample through a 0.2-mm polycarbonate membrane filter. The membrane filter is stained with acridine orange solution. The stained filter is examined for fluorescing bacteria cells using a fluorescent microscope. The fluorescent bacteria are counted. Dilutions are taken into consideration and bacterial concentrations established. |
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Scope and Application
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This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters. Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears. |
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Applicable Concentration Range
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10,000 cells/mL |
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Interferences
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None indicated. |
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Relative Cost
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$51 to $200 |
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Sample Preparation Methods
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