ASTM: D4412: Sulfate-reducing Bacteria in Water
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Official Method Name
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Standard Test Methods for Sulfate-Reducing Bacteria in Water and Water-Formed Deposits |
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Current Revision
| 1984 |
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Media
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WATER |
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Instrumentation
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Tube Culture |
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Method Subcategory
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Microbiological |
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Method Source
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Citation
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Brief Method Summary
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Water and water deposit samples and dilutions of these samples are dispensed into tubes of Starkey's medium (A or B) following five tube MPN procedures. The tubes are sealed with liquid paraffin, and incubated at 20oC for 21 days. Positive reactions are indicated by the deposit of a black precipitate. |
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Scope and Application
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These test methods cover the procedure for the detection and enumeration by the most probable number (MPN) technique of sulfate-reducing bacteria in water or water-formed deposits. Two media preparations are provided. Medium A which is prepared with reagent grade water, and Medium B which is prepared using the water to be sampled as the water source. Medium B is offered for those special conditions where sulfate-reducing bacterial strains have adapted to atypical non-fresh water environment. |
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Applicable Concentration Range
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None. |
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Interferences
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None indicated. |
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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