DOE: OH100R: TCLP Acidic Semivolatiles Using HPLC

Summary
Analytes
Revision
Data and Sites

Official Method Name

Direct Analysis of TCLP Acidic Semivolatile Compounds in Radioactive Liquid Wastes or Leachates using HPLC with Ultraviolet Absorbance Detection

Current Revision

1997

Media

OTHER

Instrumentation

High Performance Liquid Chromatography with Ultraviolet Detection

Method Subcategory

Organic

Method Source

DOE

Citation

DOE Methods

Brief Method Summary

An aqueous liquid waste or TCLP leachate is directly injected into a reverse-phase high performance liquid chromatography (HPLC) column and are eluted and detected using ultraviolet (UV) absorbance. Components are identified by their retention time on the primary column and are confirmed by either their UV-visible spectrum or their retention time on the secondary column.

Scope and Application

This method determines the toxicity characteristics leaching procedure (TCLP) acidic semivolatile compounds and certain herbicides in leachates and aqueous liquid-waste samples.

Applicable Concentration Range

0.5 - 350ug/L* (* Limits differ for each compound. This range is taken from the lowest and highest limit of the compounds listed in the method.)

Interferences

(A) UV-absorbing compounds: UV-absorbing compounds that co-elute with target analytes can interfere. (B) Alkalinity: Highly alkaline samples may degrade target analytes or interfere with chromatographic separation.

Quality Control Requirements

Example QC requirements are outlined in EPA SW-846 Methods.

Sample Handling

Sample collection, preservation, and handling should be addressed in the planning process.

Maximum Holding Time

Relative Cost

$201 to $400

Sample Preparation Methods

This method has 13 analytes associated with it.

Analyte	Detection Level	Bias	Precision	Spiking Level
2,4,5-T(93-76-5) 2,4,5-T 2,4,5-T Acid 2,4,5-Trichlorophenoxyacetic acid 2,4,5-trichloro-Acetic acid Acetic acid, (2,4,5-trichlorophenoxy)- Dacamine 4D Weedone	1.300 mg/L	89% Rec (SL)	95.00	5.00 mg/L
2,4,5-Trichlorophenol(95-95-4) 2,4,5-Trichlorophenol Dowicide 2 Phenol, 2,4,5-trichloro-	2.200 mg/L	96% Rec (SL)	95.00	5.00 mg/L
2,4,6-Trichlorophenol(88-06-2) 2,4,6-Trichlorophenol Dowicide 2S Phenachlor Phenol, 2,4,6-trichloro-	0.460 mg/L	128% Rec (SL)	95.00	2.00 mg/L
2,4-D(94-75-7) 2,4-D 2,4-D Acid 2,4-D [acetic acid, (2,4-dichlorophenoxy)-1] 2,4-Dichlorophenoxyacetic acid, salts and este 2,4-Dichlorophenoxyacetic acid, salts and esters Acetic acid, (2,4-dichlorophenoxy)- Hedonal Trinoxol Weed-b-gone	0.590 mg/L	91% Rec (SL)	95.00	5.00 mg/L
Herbicides(E-12839) Herbicide formulations Herbicides	N/A	N/A	N/A
Organics, semivolatile(E-12884) Organics, semivolatile Semi-volatile organics Semivolatile organics	N/A	N/A	N/A
Organochlorine pesticides(E-12851) Organochlorine pesticides Pesticides, organochlorine	N/A	N/A	N/A
Pentachlorophenol(87-86-5) PCP Penchlorol Penta Pentachlorophenol Phenol, pentachloro- Santophen I	3.700 mg/L	96% Rec (SL)	95.00	10.00 mg/L
Silvex(93-72-1) 2,4,5-TP 2,4,5-TP Acid 2,4,5-Trichlorophenoxypropionic acid 2-(2,4,5-Trichlorophenoxy)propionic acid Fenoprop Propanoic acid, 2-(2,4,5-trichlorophenoxy)- Propionic acid, 2-(2,4,5-trichlorophenoxy)- Silvex Silvex (ANSI)	0.810 mg/L	98% Rec (SL)	95.00	1.00 mg/L
Volatile organic compounds(E-12419) Organic compounds, volatile VOCs Volatile organic compounds	N/A	N/A	N/A
m-Cresol(108-39-4) 3-Cresol 3-Methylphenol Phenol, 3-methyl- m-Cresol m-Cresylic acid m-Hydroxytoluene m-Methylphenol m-Oxytoluene	0.900 mg/L	102% Rec (SL)	95.00	10.00 mg/L
o-Cresol(95-48-7) 1-Hydroxy-2-methylbenzene 2-Cresol 2-Hydroxytoluene 2-Methylphenol Phenol, 2-methyl- o-Cresol o-Cresylic acid o-Hydroxytoluene o-Methylphenol o-Oxytoluene	2.600 mg/L	89% Rec (SL)	95.00	5.00 mg/L
p-Cresol(106-44-5) 1-Hydroxy-4-methylbenzene 4-Cresol 4-Hydroxytoluene 4-Methylphenol Phenol, 4-methyl- p-Cresol p-Cresylic acid p-Methylhydroxybenzene p-Oxytoluene p-Tolyl alcohol	2.100 mg/L	102% Rec (SL)	95.00	10.00 mg/L

Precision Descriptor Notes:	Average recoveries and confidence intervals (CIs) are based on three replicate measurements of analytes in TCLP Leaching Fluid. Additional data for different concentrations and matrices is provided in the method. NOTE: m+p-cresol are determined together.
Detection Level Note:	Detection Limits (DLs) were calculated according to : Hubaux, A. and G. Vos. 1970. "Decision and Detection Limits for Linear Calibration Curves." Anal. Chem., 42:849-855. NOTE: Listed DLs were obt

Revision	PDF/Link
1997

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DOE: OH100R: TCLP Acidic Semivolatiles Using HPLC

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