IDEXX: IDEXX EasyDisc R2A Test Kit:  The EasyDisc R2A method is used for the quantification of culturable, waterborne bacteria. Culturable is defined as any aerobic bacteria able to form colonies on solid media.

  • Summary
  • Analytes
  • Revision
  • Data and Sites
Official Method Name
 IDEXX EasyDisc* R2A Test
Current Revision
Initial Submission
Media
 WATER
Instrumentation
 Plate Count
Method Subcategory
 Microbiological
Method Source
  IDEXX
Citation
  Standard Methods Online - Standard Methods for the Examination of Water and Wastewater
Brief Method Summary
 1. Remove white lid and add 1±0.1 mL water sample directly to the clear EasyDisc R2A plate. 2. Gently swirl immediately after sample addition to coat the plate entirely and reattach white lid. 3. Incubate undisturbed at room temperature for at least 20 minutes to allow for the media to set. Transfer plate to incubator within 1 hour of sample addition. 4. Incubate the plate, white lid side up, at 20-28°C for 5 to 7 days. 5. Examine the plates for colony growth as soon as they are removed from the incubators. Reject any plate with confluent growth. 6. Sum all colonies for the result. The result is expressed as colony forming units (CFU) per milliliter. NOTE: Most microorganisms will produce a blue color on EasyDisc, but some may produce natural pigments with colors other than blue. All colonies should be summed for the final result.
Scope and Application
 Quantification of heterotrophic bacteria in drinking water, process water, bottled water, and other non-potable waters.
Applicable Concentration Range
 <300 CFU/mL before dilution.
Interferences
Quality Control Requirements
Sample Handling
Maximum Holding Time
Relative Cost
Sample Preparation Methods
WHO WE ARE
  • NWQMC
  • USGS
  • EPA
LEGAL