Abraxis: 520011SAES: Microcystins and Nodularins by Streptavidin-amplified ADDA Immunoassay, Microtiter Plate
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Official Method Name
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Abraxis Microsystins/Nodularins (ADDA) SAES (Streptavidin Amplified Enhanced Sensitivity), ELISA Kit (96T) PN 520011SAES |
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Current Revision
| Microcystins ADDA SAES ELISA Microplate Method |
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Media
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WATER (Waterbody type - Lake) |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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The test is an indirect competitive ELISA for the congener-independent detection of Microcystins and Nodularins. It is based on the recognition of Microcystins, Nodularins, and their congeners by specific biotinylated antibodies. Toxin, when present in a sample, and a Microcystins-protein analogue immobilized on the plate compete for the binding sites of the biotinylated anti-Microcystins/Nodularins antibodies in solution. The plate is then washed and a streptavidin-HRP is added. After a second washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run. |
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Scope and Application
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The Abraxis Microcystins-ADDA SAES ELISAis an enhanced sensitivity immunoassay for the quantitative and sensitive congener-independent* detectionof Microcystins and Nodularins inwater samples. This test is suitable for the quantitative and/or qualitative detection of Microcystins and Nodularins in water samples [please refer to the appropriate technical bulletins for sample collection, handling, and treatment of drinking (treated and untreated)and recreational water samples]. If necessary, positive samples can be confirmed by HPLC, protein phosphatase assay, or other conventional methods. |
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Applicable Concentration Range
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0.05 - 5.0 |
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Approximately one year at 4 degrees |
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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