CDPHE: PIBO-EMP 2012 Invert:  Macroinvertebrate sample collection

  • Summary
  • Analytes
  • Revision
  • Data and Sites
Official Method Name
Sampling Protocol for Stream Macroinvertebrates
Current Revision
WATER  (Waterbody type - Wadeable stream)
Invertebrate Net (w/ handle)
Method Subcategory
Method Source
  Archer, Eric K.; Scully, Rebecca A.; Henderson, Richard; Roper, Brett B. and Heitke, Jeremiah D., 2012 Sampling Protocol for Stream Channel Attributes. PACFISH/INFISH Biological Opinion Effectiveness Monitoring Program (PIBO-EMP). Logan, UT. 125 p.
Brief Method Summary
The report outlines steps for macroinvertebrate sample collection of streams in the Pacific Northwest United States.
Scope and Application
The PACFISH/INFISH Effectiveness Monitoring (PIBO-EM) Program for aquatic and riparian resources was developed in 1998 in response to monitoring needs addressed in the Biological Opinions for bull trout (USFWS 1998) and steelhead (NMFS 1995). The primary objective is to determine whether priority biological and physical attributes, processes, and functions of riparian and aquatic systems are being degraded, maintained, or restored in the PIBO-EM area. The program samples within the interior Columbia River basin on lands managed by U.S. Forest Service (FS) Regions 1, 4, and 6 and the Idaho and Oregon/Washington State Offices of the Bureau of Land Management (BLM).

This document describes the sampling methods used by the PIBO-EM program during 2012. The methods are a result of 12 years of use, evaluation, and peer review. We worked with the Aquatic and Riparian Effectiveness Monitoring Program to standardize methods, which resulted in a document titled “Effectiveness Monitoring for Streams and Riparian Areas within the Pacific Northwest: Stream Channel Methods for Core Attributes” (USDA 2006). The PIBO-EM protocol incorporates all methods described in this document.
Applicable Concentration Range
Follow the guidelines for choosing collection sites.
Quality Control Requirements
Sample Handling
Preserve with 95% ethanol.
Maximum Holding Time
Relative Cost
Sample Preparation Methods
Samples must be filtered.