Beacon: 20-0020:  T-2 toxin in grains by immunoassay, Microtiter Plate

  • Summary
  • Analytes
  • Revision
  • Data and Sites
Official Method Name
T-2 Toxin Plate Kit
Current Revision
2014
Media
AGRICULTURAL PRODUCTS
Instrumentation
Immunoassay
Method Subcategory
Biotoxin
Method Source
  Beacon
Citation
  Beacon Analytical Systems Instructional Booklet
Brief Method Summary
T-2 toxin is extracted from a ground sample by shaking with methanol/water. The extract is then filtered and the extract is tested in the immunoassay. T-2 toxin-HRP enzyme conjugate is pipetted into the test wells followed by calibrators or sample extracts. T-2 toxin antibody is then pipetted into the test wells to initiate the reaction. During the 10 minute incubation period, T-2 toxin from the sample and T-2 toxin-HRP enzyme conjugate compete for binding to T-2 toxin antibody which, in turn, binds to the test well. Following this 10 minute incubation, the contents of the well are removed and the wells are washed to remove any unbound enzyme-labeled toxin. A clear substrate is then added to the wells and any bound enzyme-toxin conjugate causes the conversion to a blue color. Following a 5 minute incubation, the reaction is stopped and the amount of color in each well is read. The color of unknown samples is compared to the color of the calibrators and the T-2 toxin concentration of the samples is derived.
Scope and Application
This method determines T-2 toxin in grains.
Applicable Concentration Range
25 - 500
Interferences
Quality Control Requirements
Sample Handling
Maximum Holding Time
Relative Cost
Unknown
Sample Preparation Methods