Beacon: 20-0016: Vomitoxin (Deoxynivalenol: DON) in grains by immunoassay, Microtiter Plate with high sensitivity
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Official Method Name
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DON Plate Kit |
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Current Revision
| 2010 |
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Media
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AGRICULTURAL PRODUCTS |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Biotoxin |
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Method Source
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Citation
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Brief Method Summary
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DON is extracted from a ground sample by shaking with water. The aqueous extract is then filtered and the extract is tested in the immunoassay. DON-HRP enzyme conjugate is pipetted into test wells followed by calibrators or sample extracts and DON Antibody Solution. During the 10-minute incubation period, DON from the sample and DON-HRP enzyme conjugate compete for binding to DON antibody, which is bound to the test well. Following this 10-minute incubation, the contents of the well are removed and the wells are washed to remove any unbound toxin or enzyme-labeled toxin. A clear substrate is then added to the wells and any bound enzyme-toxin conjugate causes the conversion to a blue color. Following a 5-minute incubation, the reaction is stopped and amount of color in each well is read. The color of unknown samples is compared to the color of the calibrators and the DON concentration of the samples is derived. |
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Scope and Application
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This method determines vomitoxin (Deoxynivalenol: DON) in grains. |
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Applicable Concentration Range
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0.2 - 2.5 ppm |
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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