EPA-OW: NRSA Inverts 2009/2013 (Boat): Benthic invertebrate sample collection in large non-wadeable rivers
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Official Method Name
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Section 6: Non-Wadeable Rivers-Benthic Macroinvertebrates (of National Rivers and Streams Assessment: Field Operations Manual (2013)) |
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Current Revision
| April 2013 |
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Media
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WATER (Waterbody type - Non-wadeable stream) |
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Instrumentation
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Invertebrate Net (w/ handle) |
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Method Subcategory
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Population/Community |
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Method Source
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Citation
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Protocol
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EPA-841-B-12-009 - National Rivers and Streams Assessment: Field Operations Manual (2013) |
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Brief Method Summary
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This method is in USGS BioData as BETB for transect benthos. Collect benthic macroinvertebrate composite samples using a D-frame net with 500 um mesh openings. Take the samples from the sampling stations at the 11 transects equally distributed along the targeted reach. Composite all sample material and field-preserve with about 95% ethanol. This method is essentially the same in both the 2013 NRSA protocol document and the 2009 NRSA protocol document (USEPA. 2009. National Rivers and Streams Assessment: Field Operations Manual. EPA-841-B-07-009. U.S. Environmental Protection Agency, Washington, DC. 354 p.) |
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Scope and Application
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Methods described in this document are to be used specifically in work relating to the National Rivers and Streams Assessment. This document contains proceedures for how to collect benthic macroinvertebrate samples. |
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Applicable Concentration Range
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Interferences
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Completely fill the jar with 95% ethanol (no headspace). It is very important that sufficient ethanol be used, or the organisms will not be properly preserved. Existing water in the jar should not dilute the concentration of ethanol below 70%. Rinse net thoroughly before sampling next location. |
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Quality Control Requirements
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Collect all benthic samples at non-wadeable sites from the dominant habitat type within the 10 m x 15 m randomly selected sampling station at each transect. |
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Sample Handling
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Follow Table 6.2 for the proceedure on how to make composite sample. Replace the cap on each jar. Slowly tip the jar to a horizontal position, then gently rotate the jar to mix the preservative. Do not invert or shake the jar. After mixing, seal each jar with plastic tape. |
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Maximum Holding Time
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Relative Cost
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Unknown |
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Sample Preparation Methods
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