EPA-OW: NRSA Fecal 2009 (Wade):  Fecal Indicator sampling in small wadeable streams

  • Summary
  • Analytes
  • Revision
  • Data and Sites
Official Method Name
Section 6.6: Wadeable streams-Fecal Indicator (Enterococci) (of National Rivers and Streams Assessment: Field Operations Manual (2009))
Current Revision
2009
Media
WATER  (Waterbody type - Wadeable stream)
Instrumentation
Field Equipment
Method Subcategory
Microbiological
Method Source
  EPA-OW
Citation
  National Rivers and Streams Assessment: Field Operations Manual (2009)
Protocol
EPA-841-B-07-009 - National Rivers and Streams Assessment: Field Operations Manual (2009)
Brief Method Summary
Collect a fecal indicator sample at the last transect after all other sampling is completed. Samples must be filtered and the filters must be frozen within 6 hours of collection. Use a pre-sterilized, 250 ml bottle and collect the sample approximately 1 m off the bank at about 0.3 meter (12 inches) below the water surface. Add the sodium thiosulfate tablet, cap, and shake bottle 25 times. Following collection, place the sample in a cooler, chill for at least 15 minutes, and maintain on ice prior to filtration of four 50mL volumes.
Scope and Application
Methods described in this document are to be used specifically in work relating to the National Rivers and Streams Assessment. This document contains proceedures for how to collect a fecal indicator sample.
Applicable Concentration Range
Interferences
Follow the guidelines for choosing collection site.
Quality Control Requirements
Collect sample in a pre-sterilized, 250 ml sample bottle
Sample Handling
Approach the sampling location slowly from downstream or downwind. Collect sample approximately 1 m from bank and 0.3 m (1 foot) deep. Add sodium thiosulfate tablet to preserve sample.
Maximum Holding Time
Relative Cost
Unknown
Sample Preparation Methods
Samples must be filtered and frozen on dry ice within 6 hours of collection.