USGS-NAWQA: BS0331:  Benthic Macroinvertebrates: Preparing Samples for Processing

  • Summary
  • Analytes
  • Revisions
  • Data and Sites
Official Method Name
Preparing Benthic Macroinvertebrate Samples for Processing
Current Revision
2011
Media
Biological  (Waterbody type - Non-wadeable stream)
Instrumentation
Standard metal sieve
Method Subcategory
Population/Community
Method Source
  USGS-NAWQA
Citation
  Moulton, S.R., II, Kennen, J.G., Goldstein, R.M. and Hambrook, J.A., 2002, Revised protocols for sampling algal, invertebrate, and fish communities as part of the National Water-Quality Assessment Program: U.S. Geological Survey Open-File Report 02-150, 75 p.
Protocol
USGS NAWQA 2002 Eco Protocols - Revised Protocols for Sampling Algal, Invertebrate, and Fish Communities as Part of the National Water-Quality Assessment Program
Brief Method Summary
The goal of this method is to standardize procedures used for the taxonomic identification of benthic macroinvertebrates (BMIs). These procedures can be applied to any BMI sample submitted to the National Water Quality Laboratory (NWQL) for analysis. Taxonomic identification of BMIs requires experienced personnel trained in zoological taxonomic principles and possessing a broad knowledge of all aquatic macroinvertebrate groups. The below steps summarize the sample processing steps. See method for complete details. 1. Sample washing and sieving 2. Swirl and pour sample flotation 3. Sample preservation
Scope and Application
These procedures are used by anyone responsible for the laboratory processing of BMI samples. General Scope of Procedures—Laboratory preparation of a benthic macroinvertebrate (BMI) sample involves washing and sieving procedures to clean the sample prior to sorting organisms from the sample matrix. Preparation procedures described herein can be applied to BMI samples collected in the field using quantitative, semiquantitative, or qualitative sampling methods. These procedures do not include some method-specific preparation steps (for example, size-fractionation in qualitatively processed samples).
Applicable Concentration Range
Interferences
Sample washing is ineffective when too much material is washed at one time, especially if the sample matrix consists of fine detritus or silt. Wash smaller portions of the sample separately and then recombine in a wash basin or another sieve.
Quality Control Requirements
Sample Handling
Maximum Holding Time
Relative Cost
Unknown
Sample Preparation Methods
see method documentation PDF