Abraxis: 52254BA: Tetracyclines by Immunoassay, Microtiter Plate
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Official Method Name
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Abraxis Tetracyclines/Epimers ELISA Kit (96T) PN 52254BA |
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Current Revision
| Tetracyclines ELISA Microplate Method |
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Media
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WATER (Waterbody type - Lake) |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Organic |
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Method Source
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Citation
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Brief Method Summary
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The test is a direct competitive ELISA based on therecognition of Tetracyclines by specific antibodies. Tetracyclines, when present in a sample and a Tetracyclines-enzyme conjugate compete for the binding sites of anti-Tetracyclines antibodies which are immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Tetracyclines present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run. |
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Scope and Application
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Applicable Concentration Range
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0.11 to 0.89 |
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Interferences
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Quality Control Requirements
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Sample Handling
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Maximum Holding Time
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Approximately one year at 4 degrees |
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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