EPA-OW: T2 Myco: Mycotoxin (T-2) by LC/APCI-MS
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Official Method Name
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Development of Liquid Chromatography/Time-of-flight Mass Spectrometric Method for the Simultaneous Determination of Trichothecenes, Zearalenone, and Aflatoxins in Foodstuffs |
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Current Revision
| 2006 |
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Media
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VARIOUS |
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Instrumentation
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Liquid Chromatography-Mass Spectrometery (also High Performance LC-MS; HPLC-MS) |
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Method Subcategory
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Microbiological |
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Method Source
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Citation
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Rapid Communications in Mass Spectrometry. 20(9): 1422 - 1428 |
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Brief Method Summary
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A LC/APCI-MS procedure based on time-of-flight mass spectrometry (TOFMS), with a real-time reference mass correction, is used for simultaneous determination of Fusarium mycotoxins (to include DAS and T-2 mycotoxin) in foodstuffs. Mycotoxin samples are extracted with acetonitrile/water (85:15) and centrifuged, and the supernatant is applied to a column for cleanup. Prepared samples are separated by liquid chromatography with an aqueous mobile phase of ammonium acetate and methanol detection is provided in exact mass chromatograms with a mass window of 0.03 Th. The limits of detection range from 0.1 to 6.1 ng/g in analyzed foodstuffs. |
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Scope and Application
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A LC/APCI-MS procedure based on time-of-flight mass spectrometry (TOFMS), with a real-time reference mass correction, is used for simultaneous determination of Fusarium mycotoxins (to include DAS and T-2 mycotoxin) in foodstuffs. |
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Applicable Concentration Range
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0.1 to 6.1 ng/g |
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Interferences
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Quality Control Requirements
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SAM lists these procedures for confirmatory analysis in aerosol, solid, particulate, liquid, and water samples. Further research is needed to develop and standardize the procedures for environmental sample types. |
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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