AOAC: 993.06: Staphylococcal Enterotoxins by Enzyme Immunoassay
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Official Method Name
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AOAC Official Method 993.06: Staphylococcal Enterotoxins in Selected Foods |
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Current Revision
| 1994. 16th Edition, 4th Revision; Vol. I. |
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Media
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WATER |
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Instrumentation
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Immunoassay |
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Method Subcategory
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Biotoxin |
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Method Source
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Citation
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Brief Method Summary
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This method is an enzyme immunoassay (EIA) using a mixture of high-affinity capture antibodies for identification of toxin(s) in food samples. Samples are prepared by dilution in Tris buffer, centrifugation, and filtration of the supernatant through a syringe, with adjustment to a final pH of 7.0 to 8.0. Samples are incubated in 96-well plates with the mixture of antibodies conjugated to horseradish peroxidase (HRP), and visualized with a peroxidase substrate. Assay results are determined visually or using a microtiter plate reader. Test is considered positive for staphylococcal enterotoxins if absorbance is >0.200 and is considered negative if absorbance is =0.200. Specific toxin serotypes are not differentiated. This method detects from 1.3 to 3.3 ng/mL staphylococcal enterotoxin in extracts prepared from food containing 4 to 10 ng/mL staphylococcal enterotoxin. |
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Scope and Application
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This method is for presumptive analysis of staphylococcal enterotoxins Type B in aerosol samples, and Types A and C in aerosol, solid, particulate, liquid, and water samples. Further research is needed to develop and standardize the procedures for environmental sample types. |
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Applicable Concentration Range
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1.3 to 3.3 ng/mL |
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Interferences
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May have interference by non-target compounds. |
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Quality Control Requirements
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SAM lists this method for presumptive analysis of staphylococcal enterotoxins Type B in aerosol samples, and Types A and C in aerosol, solid, particulate, liquid, and water samples. Further research is needed to develop and standardize the procedures for environmental sample types. |
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Sample Handling
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Maximum Holding Time
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Relative Cost
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Less than $50 |
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Sample Preparation Methods
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